By “synthetic compound” is meant herein any molecule which is neither In a natural way produced by the host topic (or by a matter of a similar species given that the host species) nor by another organism, in particular an organism from A further species, from One more gender, from An additional relatives, from another class or from An additional kingdom.
262、细菌噬菌体可选自长尾噬菌体科(非限制性地比如以下属：cam病毒、lika病毒、r4病毒、acadian病毒、cooper病毒、pg1病毒、pipefish病毒、rosebush病毒、brujita病毒、che9c病毒、hawkeye病毒、plot病毒、jersey病毒、k1g病毒、sp31病毒、lmd1病毒、una4病毒、bongo病毒、rey病毒、butters病毒、charlie病毒、redi病毒、baxter病毒、nymphadora病毒、bignuz病毒、fishburne病毒、phayonce病毒、kp36病毒、rogue1病毒、rtp病毒、t1病毒、tls病毒、ab18病毒、amigo病毒、anatole病毒、andromeda病毒、attis病毒、barnyard病毒、bernal13病毒、biseptima病毒、bron病毒、c2病毒、c5病毒、cba181病毒、cbast病毒、ceci病毒、che8病毒、chi病毒、cjw1病毒、corndog病毒、cronus病毒、d3112病毒、d3病毒、decurro病毒、demosthenes病毒、doucette病毒、e125病毒、eiau病毒、ff47病毒、gaia病毒、giles病毒、gordon病毒、gordtnk病毒、harrison病毒、hk578病毒、hk97病毒、jenst病毒、jwx病毒、kellezio病毒、korra病毒、l5病毒、lambda病毒、laroye病毒、liefie病毒、marvin病毒、mudcat病毒、n15病毒、nonag病毒、np1病毒、omega病毒、p12002病毒、p12024病毒、p23病毒、p70病毒、pa6病毒、pamx74病毒、patience病毒、pbi1病毒、pepy6病毒、pfr1病毒、phic31病毒、phicbk病毒、phieta病毒、phifel病毒、phijl1病毒、pis4a病毒、psa病毒、psimuna病毒、rdjl病毒、rer2病毒、sap6病毒、send513病毒、septima3病毒、seurat病毒、sextaec病毒、sfi11病毒、sfi21dt1病毒、sitara病毒、sk1病毒、slash病毒、smoothie病毒、soups病毒、spbeta病毒、ssp2病毒、t5病毒、tank病毒、tin2病毒、titan病毒、tm4病毒、tp21病毒、tp84病毒、tria病毒、trigintaduo病毒、vegas病毒、vendetta病毒、wbeta病毒、wildcat病毒、wizard病毒、woes病毒、xp10病毒、ydn12病毒和yua病毒)。
The current invention also worries a nucleic acid vector, as described higher than, to be used in in vivo supply of the nucleic acid of interest, as described higher than, into a qualified receiver bacterial mobile, reported nucleic acid of interest making a given impact on mentioned focused receiver bacterial cell,
18. The method In accordance with embodiment 17, wherein claimed conditional origin of replication is Energetic in claimed donor bacterial cell because explained donor bacterial cell expresses a rep protein, in particular a primase-helicase.
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if possible, the genetic modification doesn't integrate a phage genome or exogenous DNA into your host bacterial chromosome or endogenous plasmid(s). ideally, the genetic modification doesn't cause expression of the exogenous protein from an built-in exogenous DNA within the host bacterial chromosome or endogenous plasmid(s).
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In a particular embodiment, when stated origin of replication is derived from phage-inducible chromosomal islands (PICIs), stated conditional origin of replication is active in explained donor bacterial mobile since said donor bacterial cell expresses a rep protein, especially a primase-helicase, especially a primase-helicase of sequence SEQ ID NO: eight.
They are typically utilised to pick out for recombination occasions involving a plasmid carrying such origins as well as genome of the germs of desire.
one. A nucleic acid vector encoding a programmable nuclease, wherein reported programmable nuclease kills a targeted receiver bacterial mobile,
In A further specific embodiment, the modulating way of the invention is with the cosmetic remedy of mentioned host issue.